6DFR

CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	34.853, 59.000, 81.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.400
R-factor	0.198 *
RMSD bond length	0.020
RMSD bond angle	0.035
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.330 *
Rmerge	0.047 *
Total number of observations	41396 *
Number of reflections	7586 *
Completeness [%]	99.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	calcium acetate	1.0 (M)	0.0015ml
2	1	drop	PEG6000	50 (%(w/w))	0.0027ml
3	1	drop	NADP+DHFR	30 (mg/ml)	0.0108ml
4	1	drop	imidazole hydrochloride	10 (mM)
5	1	reservoir	PEG6000	15 (%(w/w))
6	1	reservoir	imidazole hydrochloride	10 (mM)