6DFJ

Crystal structure of anti-Zika antibody Z021 bound to DENV-1 envelope protein DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	60.540, 91.600, 187.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.510 - 2.070
R-factor	0.1862
Rwork	0.184
R-free	0.23150
RMSD bond length	0.005
RMSD bond angle	0.752
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.570	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.046	0.702
Rpim	0.022	0.339
Number of reflections	31907	14638
<I/σ(I)>	15	2.3
Completeness [%]	99.7	99.9
Redundancy	5.8	5.9
CC(1/2)	0.997	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M sodium citrate pH 4.8, 28% Jeffamine ED-2001 pH 7.0