6DF5

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	44.430, 184.580, 104.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.492 - 1.819
R-factor	0.2136
Rwork	0.211
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.011
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.492	1.880
High resolution limit [Å]	1.819	1.819
Rmerge	0.130
Rmeas	0.142
Number of reflections	38971	3741
<I/σ(I)>	7.07	1.2
Completeness [%]	99.3	96.2
Redundancy	6.1	5.6
CC(1/2)	0.997	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350