6DF1

Anti-phosphotyrosine antibody 4G10-4D5 Fab complexed with phosphotyrosine peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	62.420, 98.930, 103.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.647 - 2.300
R-factor	0.2036
Rwork	0.203
R-free	0.22150
RMSD bond length	0.011
RMSD bond angle	1.232
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.900	2.368
High resolution limit [Å]	2.300	2.300
Number of reflections	29265
<I/σ(I)>	14.5
Completeness [%]	100.0
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG3350, 0.2M potassium/sodium tartrate, and 0.1M Bir-tris propane