6DEX

Structure of Eremothecium gossypii Shu1:Shu2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28260
Spacegroup name	P 21 21 21
Unit cell lengths	37.252, 83.909, 107.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.063 - 2.100
R-factor	0.2522
Rwork	0.251
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xyn
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.220
High resolution limit [Å]	2.100	2.100
Number of reflections	34114	5674
<I/σ(I)>	11.25	2.26
Completeness [%]	97.8	93
Redundancy	3.4
CC(1/2)	0.997	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	0.1M MgCl2, 0.1M CHES pH 9.5, 25% PEG 3350