6DDT

mouse beta-mannosidase (MANBA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	80.584, 65.458, 104.414
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	46.152 - 2.100
R-factor	0.1751
Rwork	0.174
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6u
RMSD bond length	0.004
RMSD bond angle	0.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.152	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	48793
<I/σ(I)>	17.2
Completeness [%]	76.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	19-21% PEG 5000, 0.1 M HEPES