6DD7

Crystal structure of plant UVB photoreceptor UVR8 from in situ serial Laue diffraction

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	294
Detector technology	CCD
Collection date	2015-08-24
Detector	RAYONIX MX340-HS
Wavelength(s)	1-1.25
Spacegroup name	C 1 2 1
Unit cell lengths	111.200, 80.300, 190.000
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	47.333 - 2.000
R-factor	0.2574
Rwork	0.254
R-free	0.32810
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4naa
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	Precognition
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	99141
<I/σ(I)>	10.3
Completeness [%]	85.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	9.2	294	3.5 mg/mL protein, 7-10% PEG 8000, 50 mM MgCl2, and 50 mM tris buffer at pH 9.2