6DD6
Crystal structure of bacterial (6-4) photolyase PhrB from in situ serial Laue diffraction
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 294 |
Detector technology | CCD |
Collection date | 2016-02-11 |
Detector | RAYONIX MX340-HS |
Wavelength(s) | 1-1.25 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.700, 106.800, 57.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.680 - 2.300 |
R-factor | 0.1693 |
Rwork | 0.166 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dja |
RMSD bond length | 0.009 |
RMSD bond angle | 1.127 |
Data reduction software | Precognition (5.2) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 27730 | |
<I/σ(I)> | 20.9 | |
Completeness [%] | 99.3 | 96.7 |
Redundancy | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.6 | 294 | 1.7 mg/mL protein, 9-13% PEG 4000, 6% isopropanol, and 50 mM sodium citrate buffer at pH 5.6 |