6DCZ
The N-terminal domain of PA endonuclease from the influenza H1N1 virus in complex with 3-hydroxy-4-oxo-4H-pyran-2-carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-15 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 75.840, 75.840, 121.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.890 - 2.890 |
| R-factor | 0.20684 |
| Rwork | 0.200 |
| R-free | 0.27586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4awm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.330 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.117 | 0.485 |
| Number of reflections | 4826 | 773 |
| <I/σ(I)> | 12.5 | 2.5 |
| Completeness [%] | 95.7 | 99.8 |
| Redundancy | 8.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 308 | 25% PEG4000, 100 mM Tris, pH 8.0, 200 mM sodium acetate |
