6DC7

Apo Fab structure of mouse monoclonal antibody 8B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	53.440, 67.474, 240.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.918 - 1.901
R-factor	0.1848
Rwork	0.183
R-free	0.23500
RMSD bond length	0.007
RMSD bond angle	0.879
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	1.960
High resolution limit [Å]	1.900	1.900
Number of reflections	65961
<I/σ(I)>	14.5
Completeness [%]	94.8
Redundancy	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% polyethylene glycol 6000, 1 M lithium chloride, and 0.1 M citric acid pH 4.0