6DBG

Crystal Structure of VHH R303 in complex with InlB-LRR-IR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97950
Spacegroup name	P 1
Unit cell lengths	49.890, 66.950, 73.830
Unit cell angles	116.67, 97.73, 95.08

Refinement procedure

Resolution	43.201 - 1.510
R-factor	0.1614
Rwork	0.160
R-free	0.18040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dba 2uzx
RMSD bond length	0.012
RMSD bond angle	1.099
Data reduction software	xia2
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.201	1.560
High resolution limit [Å]	1.510	1.510
Number of reflections	122420
<I/σ(I)>	15.91
Completeness [%]	93.5
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	0.1 M Sodium Citrate Tribasic Dihydrate, 34 % Jeffamine ED-2001