6DB1
2.0 Angstrom Resolution Crystal Structure of N-Terminal Ligand-Binding Domain of Putative Methyl-Accepting Chemotaxis Protein from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 63 |
| Unit cell lengths | 89.226, 89.226, 65.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.210 - 2.000 |
| R-factor | 0.22438 |
| Rwork | 0.222 |
| R-free | 0.26448 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.826 |
| Rmeas | 0.084 | 0.926 |
| Rpim | 0.036 | 0.415 |
| Number of reflections | 20259 | 1009 |
| <I/σ(I)> | 28.9 | 2.04 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.6 | 4.9 |
| CC(1/2) | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | Protein: 7.0 mg/ml, 0.01M Tris HCl (pH 8.3), ATP, Mg, Screen: PACT (C5), 0.1 PCB buffer (pH 8.0), 25% (w/v) PEG 1500 |
