6D9Z

Structure of CysZ, a sulfate permease from Pseudomonas Denitrificans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000
Spacegroup name	P 63
Unit cell lengths	225.130, 225.130, 96.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.996 - 3.402
R-factor	0.247196502171
Rwork	0.245
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d79
RMSD bond length	0.010
RMSD bond angle	1.097
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	phenix.refine (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.000	3.523
High resolution limit [Å]	3.400	3.402
Rmerge	0.095	2.890
Number of reflections	37223	3708
<I/σ(I)>	22.4	1.2
Completeness [%]	100.0	96.87
Redundancy	19.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	22-30% PEG550MME, 0.1 M Na-Hepes pH 7.0