6D9L
Ternary RsAgo Complex with Guide RNA and Target DNA Containing G-A Non-canonical Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.045, 118.253, 117.827 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 41.670 - 2.600 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.25330 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.109 | 0.777 |
| Number of reflections | 55738 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 98.1 | 97.8 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.990 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |
