6D9B
Crystal structure of Corynebacterium diphtheriae UDP-galactopyranose mutase in complex with UDP-glucose (open, oxidized)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.060, 98.060, 127.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.825 - 2.200 |
R-factor | 0.1554 |
Rwork | 0.153 |
R-free | 0.19170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5br7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.830 | 38.830 | 2.270 |
High resolution limit [Å] | 2.200 | 8.800 | 2.200 |
Rmerge | 0.117 | 0.043 | 0.895 |
Rmeas | 0.119 | 0.044 | 0.912 |
Rpim | 0.022 | 0.009 | 0.173 |
Number of reflections | 60110 | 591 | 2907 |
<I/σ(I)> | 19.3 | ||
Completeness [%] | 100.0 | 98.5 | 100 |
Redundancy | 28.1 | 22.9 | 27.3 |
CC(1/2) | 0.999 | 0.999 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris, 28% PEG400, 20% isopropanol |