6D92
Ternary RsAgo Complex with Guide RNA and Target DNA Containing A-A non-canonical pair at position 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.237, 118.942, 118.559 |
| Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
| Resolution | 42.810 - 1.810 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 160184 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 93.8 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |
