6D8O

Group I self-splicing intron P4-P6 domain mutant A230U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	P 21 21 21
Unit cell lengths	74.250, 129.490, 146.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.195 - 2.801
R-factor	0.21162944016
Rwork	0.211
R-free	0.22643
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gid
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.600
High resolution limit [Å]	2.800
Number of reflections	35173
<I/σ(I)>	15
Completeness [%]	99.3
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 1000, sodium cacodylate, spermine