6D8N

Group I self-splicing intron P4-P6 domain mutant G134A/U185AA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979300
Spacegroup name	P 21 21 21
Unit cell lengths	76.650, 130.880, 146.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.142 - 3.954
R-factor	0.247275138779
Rwork	0.246
R-free	0.26551
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gid
RMSD bond length	0.003
RMSD bond angle	0.500
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.200
High resolution limit [Å]	3.950
Number of reflections	13327
<I/σ(I)>	15.6
Completeness [%]	99.5
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MPD, sodium cacodylate, spermine