6D8M

Group I self-splicing intron P4-P6 domain mutant A125U/G126U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979300
Spacegroup name	P 21 21 21
Unit cell lengths	75.600, 132.880, 146.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.200 - 3.700
R-factor	0.202500845723
Rwork	0.199
R-free	0.22937
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gid
RMSD bond length	0.005
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.200
High resolution limit [Å]	3.700
Number of reflections	16338
<I/σ(I)>	13
Completeness [%]	99.5
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MPD, sodium cacodylate, spermine