6D8K

Bacteroides multiple species beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	I 41
Unit cell lengths	213.464, 213.464, 112.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.435 - 2.650
R-factor	0.209
Rwork	0.207
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.435	2.710
High resolution limit [Å]	2.650	2.650
Number of reflections	73250
<I/σ(I)>	14.7
Completeness [%]	99.7
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Magnesium Chloride, 0.1 M MES: NaOH, pH 6.5, 10 % (w/v) PEG 4000