6D87

Structure of the Bovine p85alpha BH domain, R262T mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-10-25
Detector	RAYONIX MX300HE
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	85.499, 91.637, 93.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.795 - 2.700
R-factor	0.198
Rwork	0.196
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbw
RMSD bond length	0.003
RMSD bond angle	0.630
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.800	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.089	0.501
Number of reflections	20681	1024
<I/σ(I)>	23.3
Completeness [%]	99.3	100
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Li2SO4, 100 mM Na Cacodylate, pH 6.0