6D7U
The crystal structure of hemagglutinin from A/Guangdong/17SF003/2016 H7N9 influenza virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.125, 117.046, 119.971 |
| Unit cell angles | 90.00, 124.46, 90.00 |
Refinement procedure
| Resolution | 37.998 - 2.700 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ln6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 62809 | |
| <I/σ(I)> | 22.27 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.2M Ammonium Formate, pH 6.6, 20% PEG 3350 |
