6D6H

Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-20
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	26.993, 31.687, 33.989
Unit cell angles	88.27, 71.25, 68.50

Refinement procedure

Resolution	13.191 - 2.000
R-factor	0.128806872456
Rwork	0.126
R-free	0.19270
RMSD bond length	0.002
RMSD bond angle	0.528
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.500	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.058	0.878
Number of reflections	6475
<I/σ(I)>	18.8	9
Completeness [%]	97.1
Redundancy	2.6
CC(1/2)	0.999	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein: 10 mg/mL in water Well: 0.3 M NaNO3