6D6F

Triclinic lysozyme cryocooled to 100 K with 47% xylose as cryoprotectant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-19
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	26.956, 31.864, 33.991
Unit cell angles	88.23, 71.54, 68.51

Refinement procedure

Resolution	13.559 - 2.000
R-factor	0.1395
Rwork	0.137
R-free	0.19990
RMSD bond length	0.002
RMSD bond angle	0.549
Data scaling software	SCALA
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.560	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.058	0.162
Number of reflections	6647
<I/σ(I)>	16.9	7.8
Completeness [%]	99.2
Redundancy	2.9
CC(1/2)	0.998	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein: 10 mg/mL Well: 0.3 M NaNO3