6D5C
Structure of Caldicellulosiruptor danielii GH10 module of glycoside hydrolase WP_045175321
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-31 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.307, 98.497, 78.810 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 86.480 - 1.900 |
| R-factor | 0.18147 |
| Rwork | 0.179 |
| R-free | 0.23331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ay7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.804 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 104001 | 14807 |
| <I/σ(I)> | 14.32 | 2.11 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.03 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris, pH 8-9, 1.5-2.1 M ammonium sulfate |
