6D58

Crystal structure of a Fc fragment of Human IgG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.590, 80.678, 136.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.888 - 2.390
R-factor	0.2369
Rwork	0.234
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.012
RMSD bond angle	1.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.260	0.481
Rpim	0.144	0.452
Number of reflections	15232	467
<I/σ(I)>	16.2	1
Completeness [%]	68.1	42.5
Redundancy	3.2	1.5
CC(1/2)	0.990	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	20% PEG MME 5,000 0.1 M Bis-Tris pH 6.5