6D4E

Crystal Structure of a Fc Fragment of Rhesus macaque (Macaca mulatta) IgG1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	57.579, 64.525, 80.644
Unit cell angles	90.00, 104.55, 90.00

Refinement procedure

Resolution	42.178 - 2.800
R-factor	0.2234
Rwork	0.221
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.006
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.166	0.739
Rpim	0.113	0.579
Number of reflections	13809
<I/σ(I)>	9.7	0.8
Completeness [%]	95.2	81.7
Redundancy	2.9	2
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	20% PEG 4000 0.1 M sodium citrate pH 4.5