6D3O

Crystal Structure of Vascular Endothelial Growth Factor (VEGF8-109) with HH4, an alpha/beta-Peptide with Irregular Secondary Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 63
Unit cell lengths	101.649, 101.649, 59.988
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.290 - 3.100
R-factor	0.2192
Rwork	0.217
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vpf
RMSD bond length	0.004
RMSD bond angle	1.353
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.149	1.430
Rmeas	0.156	1.500
Rpim	0.047	0.443
Number of reflections	7198	1029
<I/σ(I)>	13	1.7
Completeness [%]	100.0	100
Redundancy	11.1	11.3
CC(1/2)	0.997	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v PEG 10000