6D38

Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	52.039, 52.039, 163.621
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.909 - 2.800
R-factor	0.1987
Rwork	0.195
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z6y
RMSD bond length	0.010
RMSD bond angle	1.414
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.133	0.526
Number of reflections	6120	290
<I/σ(I)>	12.7	2
Completeness [%]	99.4	96.7
Redundancy	8.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.05 M Ammonium sulfate, 0.05 M BIS-TRIS pH 6.5, 30% v/v Pentaerythritol ethoxylate (15/4 EO/OH)