6D38
Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.039, 52.039, 163.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.909 - 2.800 |
| R-factor | 0.1987 |
| Rwork | 0.195 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.133 | 0.526 |
| Number of reflections | 6120 | 290 |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 8.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.05 M Ammonium sulfate, 0.05 M BIS-TRIS pH 6.5, 30% v/v Pentaerythritol ethoxylate (15/4 EO/OH) |
