6D2J

Beta Carbonic anhydrase in complex with thiocyanate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	72.087, 77.206, 86.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.100
Rwork	0.173
R-free	0.23100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.300
High resolution limit [Å]	2.100	2.100
Number of reflections	14318
<I/σ(I)>	15.5
Completeness [%]	99.3
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% PEG200; 20 mM Tris-HCl; pH 7.0