6D21

Crystal structure of the FERM domain of zebrafish FARP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	35.377, 82.723, 96.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.527 - 1.999
R-factor	0.1854
Rwork	0.183
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.981
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.530
High resolution limit [Å]	2.000
Number of reflections	18882
<I/σ(I)>	22.53
Completeness [%]	98.6
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium sodium tartrate tetrahydrate and 20 % PEG3350 (w/v)