6D1T
Complex of MBD1-MBD and methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 28.574, 74.193, 138.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.100 - 2.250 |
| R-factor | 0.2382 |
| Rwork | 0.235 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | earlier version of PDB entry 6cc8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.290 | 46.290 | 2.320 |
| High resolution limit [Å] | 2.250 | 9.000 | 2.250 |
| Rmerge | 0.056 | 0.020 | 1.083 |
| Rmeas | 0.061 | 0.022 | 1.167 |
| Rpim | 0.023 | 0.009 | 0.430 |
| Total number of observations | 51311 | 762 | 4878 |
| Number of reflections | 7412 | 674 | |
| <I/σ(I)> | 20.3 | 52.5 | 1.7 |
| Completeness [%] | 99.9 | 96.9 | 100 |
| Redundancy | 6.9 | 5.3 | 7.2 |
| CC(1/2) | 0.999 | 0.999 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30% PEG-550-MME, 0.1 M magnesium chloride, 0.1 M HEPES |
