6D1S

Crystal structure of an apo chimeric human alpha1GABAA receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9756
Spacegroup name	P 1 21 1
Unit cell lengths	108.517, 264.846, 109.153
Unit cell angles	90.00, 110.46, 90.00

Refinement procedure

Resolution	37.766 - 3.200
R-factor	0.2224
Rwork	0.220
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.910	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.104	2.470
Rpim	0.041	0.961
Number of reflections	93787
<I/σ(I)>	11.4	1
Completeness [%]	99.0
Redundancy	7.3
CC(1/2)	0.996	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	277.1	100 mM 2-(N-morpholino)ethanesulfonic acid, 400mM NaSCN, 21% PEG400