6D1O
FT_5 dioxygenase apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.462, 200.326, 98.308 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 46.190 - 2.700 |
| R-factor | 0.21493 |
| Rwork | 0.213 |
| R-free | 0.24114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 67790 | 3120 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 97.6 | 89.6 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | Crystal grew from Hampton Research SaltRx screen, condition C3: 2.0M sodium formate, 0.1M sodium acetate-pH 4.6. |
