6D1N

Apo structure of Bacteroides uniformis Beta-glucuronidase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03322
Spacegroup name	C 1 2 1
Unit cell lengths	125.780, 130.275, 94.220
Unit cell angles	90.00, 100.27, 90.00

Refinement procedure

Resolution	29.605 - 2.200
R-factor	0.1412
Rwork	0.140
R-free	0.18660
RMSD bond length	0.008
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.605	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	75592
<I/σ(I)>	18.6
Completeness [%]	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% PEG 1000, 0.1M Tris pH 8.5