6D14
Zebrafish TRAP1 bound to AMPPNP and calcium in the asymmetric closed state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 91.4 |
| Detector technology | CCD |
| Collection date | 2015-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.252, 95.751, 124.546 |
| Unit cell angles | 90.00, 134.66, 90.00 |
Refinement procedure
| Resolution | 48.400 - 2.500 |
| R-factor | 0.2312 |
| Rwork | 0.230 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ipe |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.693 |
| High resolution limit [Å] | 2.500 | 2.600 |
| Number of reflections | 49472 | 4360 |
| <I/σ(I)> | 10.36 | |
| Completeness [%] | 97.6 | 97.39 |
| Redundancy | 11.3 | |
| CC(1/2) | 0.996 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 17% PEG-3350, 0.2 M sodium/potassium tartrate, 36 mM hexammine cobalt |
