6D13

Crystal structure of E.coli RppH-DapF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 41 21 2
Unit cell lengths	85.810, 85.810, 177.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.991 - 3.060
R-factor	0.1903
Rwork	0.187
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ijz
RMSD bond length	0.009
RMSD bond angle	1.074
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.250	3.270
High resolution limit [Å]	3.060	3.060
Rmerge	0.155	1.076
Number of reflections	13075	2296
<I/σ(I)>	13.9
Completeness [%]	98.9
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	15% (v/v)PEG4,000, 0.1 M Tris-HCl, pH 8.0, and 0.2 M KI