6D02

Cross-alpha Amyloid-like Structure alphaAmL, 2nd form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 2
Unit cell lengths	107.546, 107.777, 99.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.977 - 2.500
R-factor	0.2383
Rwork	0.232
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6C51 generated antiparallel bundle
RMSD bond length	0.009
RMSD bond angle	1.122
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	41301	4048
<I/σ(I)>	16.5	2.5
Completeness [%]	100.0	99.9
Redundancy	10.9	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	45% MPD, 0.6 M NaH2PO4