6CZR

The structure of amicetin bound to the 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	210.040, 449.520, 624.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.916 - 3.140
R-factor	0.2412
Rwork	0.238
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4Y4O without YfiA
RMSD bond length	0.002
RMSD bond angle	0.508
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.916	49.916	4.820
High resolution limit [Å]	3.140	21.020	4.700
Rmerge	0.329	0.109	1.196
Rmeas	0.380	0.120	1.435
Total number of observations	729273
Number of reflections	1001501	2323	14025
<I/σ(I)>	3.28	5.61	0.97
Completeness [%]	71.8	65.9	66.6
Redundancy	3.54	4.823	2.732
CC(1/2)	0.942	0.989	0.261

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	292	0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME