6CZL
Crystal structure of Medicago truncatula ATP-phosphoribosyltransferase in relaxed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.826, 195.621, 100.209 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.920 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.090 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.067 | 0.900 |
| Rmeas | 0.076 | 1.050 |
| Number of reflections | 82460 | 12719 |
| <I/σ(I)> | 15.2 | 1.8 |
| Completeness [%] | 99.1 | 95.3 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292 | 2.63 M NaCl, 100 mM Bis-Tris propane pH 6.3 |
