6CZ8
The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to arsenate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.162, 86.344, 148.221 |
| Unit cell angles | 90.00, 127.67, 90.00 |
Refinement procedure
| Resolution | 39.100 - 1.780 |
| R-factor | 0.1376 |
| Rwork | 0.137 |
| R-free | 0.16810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 1.844 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rpim | 0.034 | 0.303 |
| Number of reflections | 222359 | 22113 |
| <I/σ(I)> | 15.94 | 2.35 |
| Completeness [%] | 99.8 | 99.84 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 10 mg/ml protein, 15% PEG 2000 MME, 150 mM KSCN, 50 mM HEPES (adjusted to pH 7.5 with NaOH), 5 mM disodium arsenate, microseeded; cryo protected in 30% PEG 2000 MME, 100 mM KSCN, 1 M sodium formate, 50 mM MES (pH 6.0), 5 mM disodium arsenate |
