6CZ2
Structure of the PTK6 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 108.624, 108.624, 83.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.310 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CNX |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.310 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 1.151 | |
| Number of reflections | 13913 | 2071 |
| <I/σ(I)> | 11.3 | 2.5 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 4.8 | 4.9 |
| CC(1/2) | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 3.3 M Potassium acetate, 0.1 M bicine, pH 8, 13oC |
