6CYJ
Mycobacterium tuberculosis transcriptional regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 144.485, 144.485, 96.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.690 - 2.699 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cz6 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13rc1_2954) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.078 | 0.044 | 1.052 |
Rmeas | 0.080 | 0.045 | 1.083 |
Rpim | 0.019 | 0.011 | 0.258 |
Total number of observations | 475646 | ||
Number of reflections | 27986 | 1589 | 1414 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 97.8 | 99.7 | 100 |
Redundancy | 17 | 15.7 | 17.1 |
CC(1/2) | 0.999 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 290 | Bicine pH9.5, Sodium Acetate, Zwittergent 3-14 |