6CYA
Rotavirus SA11 NSP2 S313A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999964 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 107.370, 107.370, 150.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.745 - 2.600 |
R-factor | 0.1974 |
Rwork | 0.196 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l9v |
RMSD bond length | 0.003 |
RMSD bond angle | 0.540 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.960 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.143 | 0.973 |
Rpim | 0.040 | 0.268 |
Number of reflections | 13937 | |
<I/σ(I)> | 17.1 | |
Completeness [%] | 99.9 | |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 5 mg/mL protein, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 14.4% polyethylene glycol, 20% glycerol |