6CXH

Crystal structure of particulate methane monooxygenase from Methylomicrobium alcaliphilum 20Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.033288, 1.377602
Spacegroup name	P 63
Unit cell lengths	143.840, 143.840, 146.152
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.579 - 2.704
R-factor	0.2161
Rwork	0.213
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rgb
RMSD bond length	0.010
RMSD bond angle	1.313
Data reduction software	HKL-2000 (2.3.10)
Data scaling software	HKL-2000 (2.3.10)
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Rmeas	0.070	0.588
Rpim	0.025	0.220
Number of reflections	46989
<I/σ(I)>	40.6	11.2
Completeness [%]	99.1	92
Redundancy	9	6.4
CC(1/2)	0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.8 M AmSO4, 0.2 M MES, pH 6