6CX2

S177G Mutant of Yeast PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-12
Detector	NOIR-1
Wavelength(s)	0.97
Spacegroup name	P 21 3
Unit cell lengths	123.756, 123.756, 123.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.750 - 3.101
R-factor	0.2184
Rwork	0.217
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.750	24.750	3.210
High resolution limit [Å]	3.100	6.640	3.100
Rmerge	0.142	0.045	0.752
Rmeas	0.150	0.048	0.796
Total number of observations	109371
Number of reflections	11688	1232	1135
<I/σ(I)>	8.2	32.2	2.1
Completeness [%]	99.9	99.3	100
Redundancy	9.29	8.52	9.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	2.2M Ammonium sulfate 20% Glycerol