6CWM
Crystal structure of SpaA-SLH/G109A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.348, 65.668, 73.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.150 |
| R-factor | 0.1322 |
| Rwork | 0.131 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cwc |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 3.120 | 1.150 |
| Rmerge | 0.043 | 0.032 | 0.745 |
| Rmeas | 0.047 | 0.035 | 0.806 |
| Rpim | 0.018 | 0.014 | 0.303 |
| Total number of observations | 419434 | ||
| Number of reflections | 57497 | ||
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 96.4 | 91 | 93.5 |
| Redundancy | 7.3 | 6.4 | 6.9 |
| CC(1/2) | 0.998 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2 M ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350 |
