6CWD
Hepatitis B core-antigen in complex with scFv e13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.540, 65.100, 148.210 |
| Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
| Resolution | 19.918 - 3.330 |
| R-factor | 0.2451 |
| Rwork | 0.242 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6z 2kh2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 3.450 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Rmeas | 0.250 | 0.820 |
| Number of reflections | 30123 | 3029 |
| <I/σ(I)> | 5 | 1.5 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.960 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 12% (w/v) PEG 20000, 0.1M Imidazole pH 7.0 |
