6CW1
Crystal structure of Neurexin-1 alpha ectodomain fragment, L2-L3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.10208 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.144, 62.901, 113.061 |
Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.840 |
R-factor | 0.2233 |
Rwork | 0.222 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qcw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.508 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.010 | 2.950 |
High resolution limit [Å] | 2.840 | 6.140 | 2.840 |
Rmerge | 0.091 | 0.062 | 0.520 |
Rmeas | 0.108 | 0.073 | 0.654 |
Rpim | 0.058 | 0.037 | 0.391 |
Number of reflections | 28540 | 2996 | 2555 |
<I/σ(I)> | 15.2 | 2.2 | |
Completeness [%] | 98.4 | 99.5 | 89.1 |
Redundancy | 3.4 | 3.6 | 2.4 |
CC(1/2) | 0.994 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.9 M NaCITRATE, 0.1 M TRIS PH 8.0, 5 MM CACL2 |