6CVZ

Crystal structure of the WD40-repeat of RFWD3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32
Unit cell lengths	91.991, 91.991, 110.738
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.2146
Rwork	0.214
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished model
RMSD bond length	0.008
RMSD bond angle	1.285
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.086	0.056	0.689
Rmeas	0.099	0.065	0.792
Rpim	0.049	0.032	0.386
Total number of observations	397184
Number of reflections	96621	4747	4884
<I/σ(I)>	7.4
Completeness [%]	99.4	97.4	99.9
Redundancy	4.1	4	4.1
CC(1/2)		0.976	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	16% PEG 3500, 0.2 M Magnesium Chloride, 0.1 M Tris pH8.0